Researchers have identified a potential new biomarker for non-small cell lung cancer (NSCLC), which may help increase the efficacy of an emerging vaccine based on the MUC-1 antigen.
Charles Butts, MD
Researchers have identified a potential new biomarker for nonsmall cell lung cancer (NSCLC), which may help increase the efficacy of an emerging vaccine based on the MUC-1 antigen.
Previously untreated patients with NSCLC tumors expressing the MUC-1 mutation experienced a median progression-free survival (PFS) of 5.9 months when they received the experimental vaccine in combination with standard first-line chemotherapy. This is in comparison to a PFS of 5.1 months in patients who received placebo plus standard chemotherapy (P=.019), according to an article recently published inThe Lancet Oncology.1
Because the trial successfully met its first endpoint, it will continue on to phase III. This portion of the trial will enroll 812 patients, according to Charles Butts, MD, professor of oncology at the University of Alberta in Edmonton, Canada.
“The difference in median PFS of 0.8 months is not clinically meaningful, but the hazard ratio for progression-free survival was 0.66 for patients in the group with lower triple-positive activated lymphocytes (TrPAL) scores. This would be clinically meaningful,” explained Butts, who coauthored a comment on the trial, which appeared in the same issue ofThe Lancet Oncologyas the aforementioned article.2
Overall survival (OS) was also significantly better in those with non-squamous histology, Butts wrote.
Therapeutic vaccine strategies, which have generated much excitement in the context of checkpoint inhibitor therapies such as nivolumab for the treatment of advanced melanoma, have not yet shown benefit in NSCLC. That may be because, unlike melanoma vaccines, they have not adequately addressed “immunosuppressive mechanisms employed by tumor cells to avoid T-cell recognition,” Butts explained in his written comments.
“The success of the checkpoint inhibitors, which overcome negative or inhibitory signals on T cells, is proof of the importance of addressing tumor-induced immunosuppression,” he added.3
That being said, the current trial, dubbed TIME, investigating TG4010 immunotherapy and first-line chemotherapy for advanced NSCLC, has found “encouraging results with improvements in outcomes and minimal toxicities. This vaccine strategy and the conditions of the phase III portion of the trial optimize the chance for successful T-cell stimulation,” wrote Butts.
TrPAL Biomarker Meets Primary Endpoint
In the study, researchers put together a cohort 222 patients between April 2012 and September 2014. All patients in the study had newly-diagnosed stage IV NSCLC, and were randomized to either the novel vaccine, TG4010, combined with standard chemotherapy or placebo with standard chemotherapy. One requirement of the patients, ages 18 or older, was that they had to express the MUC1 mutation in at least half of their cancer cells and could not have a known EGFR mutation, according to the the Quoix article.
The vaccine, TG4010, is a modified vaccinia ankara expressing MUC1 and interleukin-2.
“MUC-1 provides the antigenic stimulus to induce a cellular response while the viral vector serves to potentiate the immune response. The role of interleukin-2 is to optimize presentation of antigen to T cells,” Butts wrote.
Additionally, the viral vector is designed to limit interleukin production to the injection site, which aids in reducing side effects, according to Butts.
The primary endpoint of phase IIb was the ability of a biomarker, consisting of CD16, CD56, CD69 TrPAL, to predict improved PFS in patients who received the vaccine.
Although patients with reduced measurements of the biomarker did better, the same was not true of patients with higher quantities of TrPAL. The hazard ratio for PFS in patients with lower was lower TrPAL measurements was 0.75 compared with 0.77 for those with high measurements of TrPAL, the Quoix article said.
“Previous studies suggested that the higher TrPAL patients actually did worse with vaccine and chemo versus chemo alone. This comparison suggests the high TrPAL patients are unlike to benefit from the vaccine, but don’t necessarily do worse,” explained Butts in an exclusive interview withTargeted Oncology.
Additionally, the researchers also conducted safety analyses in the intent-to-treat population. Grade three neutropenia was slightly higher in the vaccine group (26% vs 21%), as were injection-site reactions (33% vs 4%), according to the article.